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The progression of staining in the early time points reflects the relative pattern at the 18 h time end point.
The outcome measurement of insulin resistance at the final time end point is cross-sectional and reflects risk at age 14 years.
We were compelled to use this time end point in consideration of the major heterogeneity in follow-up duration from one centre to another.
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Similar calculations were attempted for the subsequent time end points (18 months and 2 years).
Therefore, predefined time end points were used as fixed censored dates for repeated analyses.
For the subsequent time end points, the proportional hazard assumption was not verified which precluded Cox model analysis.
The Cox proportional hazard model was run at different predefined time end points, chosen according to their clinical significance.
For the whole population time end points were 1 year and 18 months: for inoperable patients 1 year only, for operable patients 2 years only.
Uric acid was measured using a timed end point method on a Beckman Coulter Synchron CX9.
Serum creatinine was measured using the timed end point method (Unicel DxC 800, Beckman and Coulter, Krefeld, Germany).
Urine protein concentration was measured by the timed end point method and urine creatinine concentration was measured by the modified Jaffe method [ 31, 32].
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