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But note that, at the same time, depletion seems to harm no one.
At the same time, depletion of nhr-64 causes increased expression of acyl-CoA oxidase, which functions in peroxisomal fat oxidation.
Remarkably, depletion of endogenous Par1b impaired Dvl/Smad4 association but, at the same time, depletion of endogenous Dvl diminished Par1b/Smad4 complex formation.
As expected, stimulation of Bmi1 expression could also be achieved in ERα-restored MDA-MB-231 cells, and at the same time depletion of ERα decreased expression of Bmi1.
Animals were depleted at 6 dpi, since the time window of complete depletion is less than two weeks and we wanted to time depletion in the onset of clinical disease.
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It is observed that noon time depletions in TEC distributions predominantly occur in winter for minimum Sun Spots Numbers (SSN) in the central regions of Turkey which also exhibit high variability due to midlatitude winter anomaly.
Quantitative cell biology studies combined with real-time depletion and repletion experiments have begun to address this (later).
Our approach using DEREG mice circumvents these issues by specifically timed depletion of Treg.
To investigate the functional role of Treg in the CNS during peak EAE, we chose DEREG mice which express a diphtheria toxin (DTx) receptor-enhanced GFP fusion protein, under the control of the Foxp3 gene locus, permitting specifically timed depletion of Treg by treatment with DTx [ 6].
The tandem instrumentation used in these studies utilized two or three discrete drift tubes and time-depletion ion gates in order to conduct mobility-selective experiments.
In this configuration, the DTIMS is operated as a mobility-selective filter using timed-depletion grids (Tyndall gates) in a manner similar to early dual-gate DTIMS designs.
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