In this case, we could again see a clear time-dependent transcript accumulation pattern in response to salinity stress.
Upon a first inspection, some of clusters, despite subtle differences in their time-dependent transcript profiles, showed an overrepresentation of genes from the same functional categories.
As a proof of principle, we show that selective measuring of transcripts that are only synthesised after a perturbation [4-thiouridine (4sU) sequencing (4sU-seq)] is a more sensitive method to identify targets and time-dependent transcriptional responses than global transcript profiling.
Treatment with either CG-1521 or TSA results in the time dependent repression of transcripts associated with M phase; specifically microtubule organization (NEK2, PRC1, KIF11, KIF23, KIF2C, KIF4A, KIF4B) and is most evident after 48 h, exemplified by KIF4A (Fig. 6); changes in the remaining mRNA transcripts are shown in Table S5.
As shown in Figure 3, all three inhibitors elicited a time dependent suppression of HIF regulated transcripts, as well as variable kinetics.
Interestingly, X-ray irradiation in H157 and H446 cells seems to demonstrate time dependent and dose dependent increases of Axin transcripts, with a more significant increase noted at the 72 hour point (P<0.01) and with 2 Gy.
poxa1b showed the highest repression level of all genes evaluated, in both fermentations conducted with dyes added and seems to be the most affected by copper and/or dye among the P. otreatus laccase transcripts analyzed in this research, with this response possibly being growth time dependent.
E11 NRXN2α splicing repression was time dependent, reaching a maximum within 6 hours, and reversible, recovering baseline levels of E11-included transcripts within 6 hours after removal of the depolarizing medium.
Trip time dependent on tides.
(a) concentration dependent (b) time dependent.
This effect was time dependent.
