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The acquisition (time to achieve 95% CAR) for rats administered with the extract was dose- and time-dependent compared to control group, which took 12 days for acquisition.
The results presented in Figure 3A, show that in the case of the direct-nano treated cells, the doxorubicin present is at higher concentrations in the cells in a time dependent manner compared to conj-nano, suggesting an efficient drug entry through apotransferrin in direct-nano as compared to that observed in conj-nano.
It was found that (fig. 4B), the increase in the counts of sub diploidal (sub G1/G0) cells in a time dependent way as compared to untreated control.
ALDH1A1 and ALDH4A1 were incubated with ACVL1, ACV1, ACV2 and ACV for 30 min and the turnover was monitored for another 45 min. As all ACV derivatives are covalent and thus time dependent inhibitors we compared their IC50 values after the same incubation time.
By testing apoptosis at different time intervals, the flow cytometric analysis of HeLa and HepG2 cells treated with the IC50 of MBS extract showed that the extract provoked significant apoptosis (P < 0.05) in the treated HeLa and HepG2 cells in a time dependent manner when compared with untreated cells.
(c) Time dependent quantitative results comparing the amount of surface anchored prickly and round NDs (Error bar represents SD. Inserted numbers are p values for the comparison at each time point, n = 4).
(c) Time dependent quantitative results comparing the total amount (including both surface anchored and internalized) of prickly and round NDs, the amount of the internalized round NDs was ~4 times higher than that of the prickly NDs (Error bar represents SD. Inserted numbers are p values for the comparison at each time point, n = 4).
Operating conditions spanning several types of bifurcation are investigated and the selectivity observed from time-dependent integrations compared to those appropriate to steady-state operation.
Third, the combination of NPRL2 and cisplatin increased the G2/M population in a time-dependent manner compared with treatment of cisplatin only.
We also observed that HBx-transfected cells were induced a significant decrease in the phosphorylation of p85 and Akt in response to insulin treatment in the time-dependent manner, compared to mock-transfeced cells (Figure 6B).
The infected, but not uninfected monocytes, when subjected to short duration of physiological levels of shear stress, expressed significantly more chemokines and cytokines in a time-dependent fashion, compared to static conditions.
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