Similarly, PCR setup and downstream sequencing processes for the MIT-NGS method are comparable to the SBT method, but less laborious and time consuming since sequencing reactions are reduced due to multiplexing and pooling (Additional file 9).
Previously, presentation of a genome on the chromosome level was done only for a limited number of yeast species, and by using expensive and time consuming Sanger sequencing [ 34].
However, it is too costly, inefficient, and time consuming to sequence the entire human genome.
Compared with the traditional Sanger method, which is expensive and time consuming, next-generation sequencing (NGS) technologies or massively parallel sequencing technologies (e. g. Illumina/Solexa-based RNA-Seq technology) are much simpler and more cost-effective [ 17].
The 2 sets of primers designed for the specific PCR amplification of A. ovis and A. marginale allow easy-to-detect and discriminate between the 2 pathogens, thus avoiding the time-consuming sequencing or multi-gene amplification procedures.
We conducted an RFLP assay in order to determine 7-C carrying individuals in mtDNA D310 region by eliminating the time-consuming sequencing method.
The present molecular authentication methods, such as AS-PCR, DNA sequence analysis, and SCAR markers, often require screening new fragments or alleles to design specific primers when new adulterants are encountered; thus, these techniques require time-consuming sequencing or have limited application.
In addition, the high cost, limited sensitivity and time consuming nature of sequencing has prompted the development of alternative methods that are more cost effective, faster, easier to perform, and more sensitive.
While RE selection may be time consuming for some sequences, the same combination of RE may be used for all subsequent sequencings.
Besides being time consuming, analyzing every sequence is also not an ideal procedure (especially in this case) given the following observation.
