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Previous studies have used time consuming sample preparation methods (offline solid phase extraction/derivatization) or methods with rather high detection limits (direct injection) for sucralose analysis.
Drawbacks of derivatization, namely incomplete reaction and time consuming sample preparation, have been avoided and the change from SIM to MRM mode resulted in increased sensitivity and lower LOQ.
Its advantages over PCR-based techniques are even shorter reaction time, no need for specific equipment, high sensitivity and specificity as well as comparably low susceptibility to inhibitors present in sample materials which enables detection of the pathogens in sample materials even without time consuming sample preparation.
Unfortunately, due to work and time consuming sample preparation, the measurement of cellulose molecular weight distributions has a limited applicability until now.
The ideal detection system would integrate sample processing and pathogen characterization into a single automated device that would eliminate laborious, and time consuming sample processing and costly detection.
Overall, this method owns some pitfalls which are primarily due to: i) time consuming sample processing and ii) relatively low abundance of DNA isolated from colonies, iii) culture conditions (i.e. Epo +/−) may alter the proportion of colonies bearing the mutation.
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Since simulation is often time consuming, sampling as few design points from the design space as possible is desired.
We use here the same SVM-regression procedure as RNAz [ 15] to estimate the z-scores from the sequence composition to avoid the time consuming sampling of shuffled alignments.
Both methods are able to ensure global coverage of the metabolome; however, these methodologies are time consuming in sample preparation and analysis.
In vitro MRS – It provides a far superior spectral resolution to either HR-MAS or in vivo MRS but is invasive and more time consuming in sample preparation than HR-MAS.
Not only is it time consuming to sample more than one room, but researchers are typically interested in focusing on the room that accounts for the highest proportion of the child's exposure.
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