The first two categories rely on labor-intensive and time-consuming restriction digestion and ligation of genomic DNA before PCR amplification (Rosenthal and Jones 1990; Acevedo et al. 2008; Leoni et al. 2008, 2010; Trinh et al. 2012b; Spalinskas et al. 2013).
But if you ban these tests, or effectively eliminate them for most people by imposing expensive and time-consuming restrictions, how does that help the public?
Global interest in the case, which ran for 17 months over the course of the two trials, has been high, partly because discovery of the plot in August 2006 led to worldwide, time-consuming restrictions at airport security checkpoints that are still in place on the liquids and creams passengers can carry aboard aircraft.
The main reason is that detecting interaction effects with adequate power usually requires much larger sample sizes than detecting main effects [ 3, 4], consequently making the study infeasible or unduly time consuming in the face of resource restrictions.
Detection of sex-specific restriction patterns [ 8] requires some pre-analysis development (i.e. sequencing to identify restriction sites) and enzyme restriction of PCR products, which is time consuming.
It also eliminates the time consuming process of isolating plasmid DNA, performing a restriction digest, and transforming E. coli with the ligated plasmid.
Furthermore, determination of the true boundaries of direct repeats by traditional methods of restriction mapping and direct sequencing is laborious and time consuming [ 47].
"While the upload process can be a bit time consuming, that's a small one-time price to pay for sidestepping expensive licensing bills and onerous restrictions.
Conventional genotyping methods such as allele-specific PCR and restriction fragment length polymorphism have been criticized for their poor sensitivity and specificity, are labour-intensive and time consuming.
Cooking is time consuming".
Time consuming drive this.
