Exact(2)
Sequencing genomic DNA using primers identified from ESTs proved to be an effective but time consuming methodology of SNP identification in rainbow trout, therefore not suitable for high throughput SNP discovery.
For wheat, reverse genetics was not so "easy", and whilst stable transformation with RNAi constructs was being developed it was, and remains, a relatively time consuming methodology, which had not really addressed many pathogenic interactions.
Similar(57)
A simple, non-time-consuming methodology is proposed.
Delphi studies, thus, have often been considered a relatively time-consuming methodology [22].
The need to strictly follow this sometimes costly and time-consuming methodology has been questioned [30, 31].
Moreover, the removal operation can be easily carried out by means of an organic solvent following a cost-effective and low time-consuming methodology.
Once developed, such less-time-consuming methodology to determine TCR complexity may have the potential to become a tool for early treatment stratification in children with SAA, if confirmed by other study groups.
In practice, the examples of published data on information content in Table 1 show that even with time-consuming methodology, including a high number of replicates and many beats measured per replicate, information content can still be low.
Kinematic techniques have been used in pigs to study the effect of different flooring types on locomotion [ 10, 11] and to quantify lameness in sows [ 12], although this rather complicated, expensive and time-consuming methodology is unlikely to be extrapolated to a practical situation.
A numerical comparison between a one-by-one simulation-based forecast and the proposed aggregated approach indicates that no significant discrepancies exists, validating and suggesting the use of the less time consuming proposed aggregated methodology.
The latter finding is of particular important, since it supports the notion that EPCs obtained by labor intensive and time consuming cell culture methodology, thus exhibiting several functional properties of endothelial cells, are indeed related to vascular biology, and are not monocytic cells that may also be involved in inflammation.
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