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Genotyping analysis was measured without amplification measuring steps because of its time-consuming disadvantage.
In this study, fluorescence intensity was measured at melting analysis step instead of amplification step because of its time-consuming disadvantage.
However, Dean and Barber's tool [ 11] requires four reviewers to rate error severity in order to achieve acceptable reliability, which could potentially be viewed as time consuming and a disadvantage of that particular tool.
However, disadvantages like time consuming make it necessary to develop a new highly efficient method.
The other disadvantages include time consuming and host resistance because of lysogen formation.
Direct tests like the secretin-CCK or secretin-caerulein test have the highest sensitivity and specificity for the detection of exocrine pancreatic insufficiency, and they remain the gold standard for testing pancreatic exocrine function but they have various practical disadvantages being time consuming, invasive and expensive.
Both these methods have the disadvantage of being time consuming and require an experienced technician for the evaluation of the result, whereas the optical densities of the ELISA can be measured photometrically.
Conventional and reliable techniques have been optimized and practiced since decades but suffer with the disadvantages of being time consuming, expensive and may be unsuitable at times [6].
However, these methods have several disadvantages, including long time consuming poisonous residual solvents and low recoveries [ 17].
However, this approach has two main disadvantages: it is time consuming and the estimated distribution will depend on the length of follow-up [ 34].
MR arthrography has the disadvantage of being more time consuming and invasive, with some patients experiencing post-procedural pain [33, 34].
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