Sentence examples for time after plating from inspiring English sources

Total differentiation time is indicated as D7+N, where D7 (or earlier) indicates the time in suspension and N is the time after plating.

The presence of these actin-rich membrane "blebs", which we will refer to as actin foci, decreased as a function of time after plating the cells and appeared to correlate inversely to cell spreading.

To determine the region of GAP273 responsible for the formation of actin foci, we measured the number of actin foci in cells transiently expressing various fragments of ARFGAP1 fused to GFP as a function of time after plating the cells (Figure 6).

At specific points in time after plating (Days 0, 1, 2, 3, 5, 7, 11, and 13), cells were trypsinized and the number of cells was determined using a Coulter Counter (Beckman Coulter Inc. Miami, FL, U.S).

Figure 9 shows representative SEM images of early E. coli, B. diminuta and C. albicans microcolonies growing on membranes at various times after plating.

Using embryoid body culture, ESC were differentiated in the presence or absence of OHT to induce Notch signaling and analyzed at different times after plating.

We then assessed the percentage of GFP-positive cells at different times after plating at high density - conditions in which control cells fail to proliferate.

We retrovirally infected and selected pools of NSLT cells with SMDF, Ras or empty vector (Figure 2A), seeded the cells at sub-confluency and then counted cells at different times after plating (Figure 2B).

Moreover, we analyzed the growth potential of H4R-Lovo cells by WST-1 assay at different times after plating.

Cell proliferation was determined by counting the cell numbers with a haemocytometer at the indicated times after plating cells into 24-well plates for 2 h.

Senescence: cells were stained at the indicated times after plating using a Senescence Cells Histochemical Staining Kit (Sigma Aldrich), according to manufacturer's protocol.