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Consequently, some mice survived for a very long time after challenge.
At any point in time after challenge, the RL in the SGP group was at least 2-fold that in the NSGP group (P < 0.01 at 15 min).
Mice immunized with pVAX-CDPK1 (17.3 ± 4.3 days) or pVAX/IL-21/IL-15 pVAX/IL-21/IL-15 pVAX/IL-21/IL-15rolonged survival time after challenge with 12.0achyzoites of the virulent RH strain in comparison to mice in groups of pVAX I, PBS and blank control, but there was significant difference of survival time between the group of pVAX-CDPK1 and pVAX/IL-21/IL-15 (P < 0.05).
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Burkholderia-infected macrophages were lysed with 1% Triton X-100 in phosphate buffered saline (PBS) at the indicated times after challenge.
As a direct measure of the balance of immunogenic mDCs and tolerogenic pDCs in the lung, we also determined the ratio of HDM+ mDCs to HDM+ pDCs in the lung at various times after challenge.
MOMP-vault immunized mice produced Th1 cells and demonstrated the migration of Th1 cells by significantly altering the number of Th1 cells found in ILNs at different times after challenge (Fig. 8b).
Rectal bodytemperature was measured at different times after challenge.
Effects of group and time (day) were not detected in platelet counts at any time point after challenge with BVDV 1373.
For pneumococcal recovery, immunized mice were sacrificed as described above at different time points after challenge.
Pneumococcal loads in lung homogenates and blood and were determined at different time points after challenge.
We observed that at both time points after challenge the predominant responses against influenza antigen were of the IgG1 isotype (Figure 4B,C,D [18] and that the use of CT, and particularly CT-RA, enhance the IgG2a isotype to the highest levels among the TCI groups.
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