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The region III (Fig. 5d) is a tilt section indicating the fracture behavior of the individual nanopore.
Leginon incorporates tomographic and other tilt data collection protocols such as conical tilt (section 6.1).
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For the samples with thickness of more than 2 μm, it is possible to get the transverse and tilt sections, as well as cross-section, at the same specimen.
However, although the linear distribution of PLEKHA7 and ZO-1 along the apical borders of bronchial cells was very similar and partially overlapping, close inspection of appropriately tilted sections showed that the two proteins were localized in adjacent, but separate areas of the junction (Fig. 5J-K), confirming that the localizations of PLEKHA7 and ZO-1 are distinct.
(b) Tilted cross section of an inductor integrated on the PS region (SEM picture).
Figure 2 SEM images of sample B. (a) Tilted cross section and (b) a magnification showing the silver particles.
CryoET tilt cross-section slices revealed that a majority of AH vaults contained internalized mass that completely traverses the vault lumen (white arrowheads), a significant distance of >35 nm with an apparent thickness of ∼3 5 nm.
Images for spindle tilt, tissue sections, and general spindle morphology were acquired using primarily a Yokogawa CSU10 spinning disk mounted on a DM16000B inverted microscope (Leica, ×63 Plan-Apocromat NA 1.4 Oil Objective) with an Andor ILE laser launch with 50 mW Coherent OBIS lasers (405, 488, 561, and 642) unless otherwise noted in the manuscript.
The most prominent advantage of FESEM characterization on PAAs is to observe the fracture sections including transversal, tilt and cross-sections, which can reveal a great deal of microstructure information.
The Dynamic Attractions SFX coaster will feature drop- and tilt-track sections as well as 3D screens.
Two dominant failure modes are identified: (1) tilting and net section failure (T+NSF) and (2) tilting, bearing and pull out (T+B+PO).
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