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This problem is exacerbated in tomography, since many tilt images of the same specimen area must be collected, and yet each tilt image must contain sufficient information for accurate tiltseries alignment to yield a high-fidelity reconstruction.
Using a larger tilt step for cryoSPT also has the positive effect that each tilt image has a higher dose, permitting the application of some methods that require high contrast in each image of a tiltseries (Iwasaki et al. 2005; Bartesaghi et al. 2012; Zhang and Ren 2012).
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Each volume reconstruction is stored with a full set of descriptors along with tilt images and any derived products such as segmented objects and animations.
Unfortunately, the broad adoption of this powerful approach has been limited by the practical challenges inherent in manual data collection of the required pairs of matching high and low tilt images (typically 60° and 0°).
The system includes a feature for montaging untilted images to ensure that all of the particles in the tilted image may be used in the reconstruction.
The microscopist is obliged to keep the imaging area centered during tilting as well as to maintain accurate focus in the tilted image while minimizing the overall electron dose, a challenging and time consuming process.
To evaluate the transposition of the anterior superior oblique insertion as a treatment for cyclovertical diplopia accompanied by an awareness of tilted image perceived with the affected eye induced from limited macular translocation (LMT).
As the tilted image indicates, the inverted pyramidal pore arrays were reconsidered as ordered silicon pyramid arrays.
The dose for each tilted image was about 1 e/Å2, and the defocus was set to 3 μm.
Figure 1 displays a SEM tilted image of a sample irradiated with a very low electronic dose (40 mC/cm2) and immersed in a pure PBS solution for 1 h.
Figure 2 shows a SEM tilted image of PS irradiated in the same conditions with that of the one displayed in Figure 1 but immersed in a 15-μM BSA-containing buffer solution.
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