The cecum was then ligated tightly 5 mm from its base and subsequently punctured once with a sterile 18-gauge needle on the anti-mesenteric border.
Since 18F-metal complexes form quickly and in many instances very tightly [13], this provided the rationale to explore a peptide-chelate conjugate for rapid radiofluorination.
Notably, enzymes catalyzing the formation of any of the other vitamers of B6, e.g. pyridoxol and pyridoxamine and their phosphorylated derivatives do not bind the products of their reactions tightly [10], [41].
The dyadic cleft is a signaling micro domain in which the Ca2+-induced Ca2+ release mechanism is controlled tightly (17, 18).
In contrast, the Δ1 25 variant bound DNA much more tightly (20- to 29-fold) than the wild-type protein in the presence of 0.15 or 1 mM Mg2+.
For cSti1, the M705V mutation bound less tightly (Kd=0.20±0.03 μM) than the wild-type protein (Kd=0.04±0.01 μM; Figures 6c and 6d).
This twisting motion changes the relationship between switch 1 and 2 and the P-loop such that Pi and ADP can no longer bind tightly (19− 22).
The starting material binds tightly (80 n M) whilst the mono-heterocyclized intermediate (mimicked by PatE2 C51P) binds more weakly (KD 1500 n M).
Screw on wire nuts/connectors to cover the exposed connections and secure them tightly.[10] These should be provided with the fixture, but if not, can be purchased cheaply at any hardware store.
Close the jar tightly.[4].
While the thread should not be excessively slack, never pull the stitches tightly.[8].
