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In this system, once tight repression is established, the only HSV-1 protein able to de-repress the quiescent genomes is ICP0.
Vietnam's Communist government is often chided for its tight repression and the absence of democracy.
The results suggest that high gene count, cooperativity of at least two, tight repression, and balanced decay rates are necessary for robust gates.
Cells grown in the presence of doxycycline showed a very tight repression of clathrin expression.
Previous observations showed that the entire hTERT gene was embedded in a highly condensed chromatin in human normal fibroblasts with a tight repression of hTERT transcription [29], [30].
Owing to the tight repression by tetR protein, in the absence of the inducers, tetracycline and sodium butyrate, HEK293S-TetR cells harboring the CD81 gene did not show detectable fluorescent signal from leaky expression of CD81 gene.
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It is not clear why Vietnam has embarked on its recent campaign of tighter repression.
"Yeltsin is their nightmare". This diplomat, who spoke on condition he not be further identified, said he believes "the more immediate effect will be tighter repression" in China.
In cases where tighter repression of gene expression is desired, synthetic biologists have had success employing multiple repression mechanisms in parallel.
This is thought to be due to tighter repression of telomerase expression in mesenchymal tissues, which have a slower cell turnover and less telomere shortening than many epithelial tissues (Henson et al, 2002).
This system was further improved by making a single amino acid substitution, W220F, in lacI which had previously been shown to strengthen LacI dimerization and provide tighter repression than wild type LacI.
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