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While many observers agree that Uganda's political row is about larger political differences, they say the tight nucleus of personalities is a steering force in the events.
In the event that Macron's vision of France stretched to the outer ring of one of those diagrams of concentric circles that kids draw to represent their position in the universe, Le Pen's was confined to its tight nucleus.
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To determine EC nuclei or tight junction number density, the EC nuclei or tight junction number was divided by the EC surface dimension as measured in microns.
"Central ring" cells displayed MLC-GFP expression in a tight ring around the nucleus or immediately adjacent to it.
In order to realize these applications through controlling protein function by optochemically triggered translocation into the nucleus, a tight photoactivatable nuclear entry mechanism is needed.
For example, ZO-1 [ 77], ZO-2 [ 78], huASH1 [ 79] and symplekin [ 45] are localized to both the tight junction and the nucleus.
One possibility is that ASPP2, upon external stimuli, may be able to shuttle from tight junctions to the nucleus to have its transcriptional role, in a similar way to other junctional proteins such as β-catenin or ZO-1.
Although SpTPC3-expressing cells also fail to accumulate cholera toxin within the Golgi, their tighter pattern of accumulation close to the nucleus suggests a transport block at a different point during endocytosis.
These scenarios suggest that the tight association of a nucleus-associated centrosome with clustered centromeres during the entire cell cycle, as in fission yeast and Dictyostelium, is primitive.
For quantitative analysis, EC nuclei number, tight junction number, pericyte cell body/process length, and EC surface length were quantified using ImageJ software (NIH) on a minimum of six images of cerebral blood vessels per sample.
Due to the tight packing of eye disc nuclei, many such segmented objects contain multiple nuclei whose fluorescence signals partially overlap.
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