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The tight borders were thrown open in 1992 and suddenly it became possible for anyone to visit.
Mike Dunafon, the mayor pro tem whose personality can hardly be constrained by the city's tight borders, suggested rugby.
Briefly, undifferentiated hESC and phESC, adherent on a feeder layer of human neonatal skin fibroblasts, were allowed to overgrow (about 7 10 days after passaging) until the colonies lost their tight borders and became multilayered, at which time bFGF was withdrawn from culture medium, inducing spontaneous differentiation.
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New Zealand has tight border controls and issues stiff penalties for any infringements.
The black population's exposure was limited then by segregation and tight border controls with some neighboring countries where H.I.V. was spreading faster.
In February Austria, Slovenia, Croatia, Serbia and Macedonia decided to impose tight border restrictions including daily caps on the number of asylum seekers and migrants allowed to enter their territory.
Louis Kuijs of RBS, an investment bank, argues that, when it comes to controlling hot money, the pilot works "only if there is a very tight border between the zone and the rest of China".
She militates against the "Islamization" of France, advocates France's withdrawal from the European Union's open-borders policy, favors tight border controls to prevent the arrival of undocumented immigrants, and advocates a vigorous policy of deportation.
Leave's victory owed much to the fact that it managed to get through the campaign without having to choose between tight border control and continued single market access.
Those alliances will make his task of forming a new government more complex in a country where support for tight border controls and the welfare state runs broad and deep.
But many growers are concerned that tight border controls will continue to cut deeply into their labor force and that, as happened last year, crops will be left to rot in the fields.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com