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Compared to higher tier risk assessments or field studies, population models do also offer the advantage that they are flexible.
Aquatic life stages occurring in permanent waters are therefore covered by the existing first tier risk assessment for fish [22].
Also, higher tier risk assessments are typically based on a limited set of data from the literature, and usually much less literature is reviewed for a risk assessment (depending on what is refined) than for a population model.
Hence, the limited knowledge of the ecology of a species affects not only the realism of model simulations, but also of higher tier risk assessments or the use of field studies in risk assessment.
However, this lack of knowledge is nothing which is specific for population models, but it is equally affecting any other higher tier risk assessments, which are based on field studies or the literature.
These investigations show that population effects in the field are a factor of 10 to 100 lower than those predicted to be safe, even by the most conservative "first tier" risk characterization in the EU.
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Short-term in vivo studies using rodent models could also be used in tier 2 level risk assessment (Table 2).
Perkins et al. (2013) described how different invertebrates could be used as candidates for tier 2 level risk assessment and provide a low-cost alternative to in vivo toxicity testing in rodents.
Finally, the relevance of pulsed dosing tests in higher-tier risk assessment of plant production products (PPP) was highlighted.
How mixture toxicity can be considered in the higher-tier risk assessment remains therefore an open question beyond the scope of the present study.
Although it may have been frustrating for Hans Toni that for a long time effect models in environmental risk assessment were only reluctantly accepted, it certainly will enjoy him that now his students play an active role in the promoting and further development of effect models as tools for higher-tier risk assessment [16 18].
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