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For example, elevation of endocannabinoid concentrations by inhibitors of their re-uptake and degradation produced a decrease in thyroid transformed cells growth [ 39].
These results contrast with the observations made by Bifulco et al. with rat thyroid transformed cells, in which tumor levels of AEA, 2-AG and PEA were all three augmented after an intratumor treatment with the FAAH inhibitor arachidonoyl-serotonin [ 39].
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Eight years later, local recurrence of thyroid cancer showed intense fluorodeoxyglucose/positron emission tomography (FDG-PET) uptake at the paratracheal region, which was suspected as a remnant tumor of the thyroid that transformed from differentiated to ATC.
Berlingieri et al (1995) demonstrated the appearance of a highly malignant phenotype in relation with HMGI-C expression in differentiated rat thyroid cells transformed with oncogenes.
TPC1 cells, which are spontaneously transformed thyroid cancer cells isolated from a patient, represent well-differentiated papillary thyroid cancer in this study.
51 As mentioned before, LPI is a GPR55 endogenous ligand and is now known to be secreted by fibroblasts and epithelial cancer cells and transformed thyroid cells, leading to mitogenic effects.
We also studied the expression of HLTF protein by immunocytofluorescence in 3 different transformed thyroid cell lines, B-CPAP, FTC-133 and 8505C, derived from human papillary carcinoma, follicular carcinoma and anaplastic carcinoma, respectively.
Indeed, the reintroduction in vitro of an exogenous Pax8 in the PCPy transformed thyroid cell line, in which Pax8 is absent as well as all the differentiation markers, was sufficient to re-activate transcription of the endogenous genes encoding thyroglobulin, thyroperoxidase and sodium/iodide symporter [ 8].
In fact, a significant increase of FRA-1 protein levels was detected in all oncogene transformed rat thyroid cell lines, and also in human thyroid carcinoma cell lines and tissues [ 12].
Thyroid hormone values were transformed by natural logarithm (ln) to obtain variables that were approximately normally distributed.
Fra-1 gene induction was required for establishment of the neoplastic phenotype in retrovirally transformed rat thyroid cells [ 31].
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