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There was thyroid tissue in the background of these biopsies confirming thyroid sampling.
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EXPERIMENTAL DESIGN: Gene expression analysis using microarray platform was performed on 6 pathologically normal thyroid samples and 12 primary follicular and papillary thyroid neoplasms.
Based on possible effects of PFCs on the thyroid, Pirali et al. [262] examined PFOS and PFOA in surgical thyroid samples from patients with diseases of the thyroid.
Microarrays containing probes for 5,760 human full-length cDNAs were used for hybridization with total RNA from normal and tumor thyroid samples labeled with Cy3-dUTP and Cy5-dUTP, respectively.
In the present study, radioactivity measurements of individual thyroid samples would have enhanced the certainty in absorbed dose calculations but was not possible since all excised thyroid tissue was needed to ensure sufficient amount of RNA for microarray analysis.
Unfortunately, all parts of the thyroid samples from mice in the three studies this work is based on were used for microarray analysis, why further studies of genomic rearrangements were not possible from the same samples.
Thyroid samples, 5 normal and 23 papillary thyroid carcinomas, were selected for this study (Table S2).
The expression of the TNC/ANXA2 pair was directly correlated (P<0.05) only in normal thyroid samples.
TGFA transcripts were significantly increased in all PTCs relative to normal thyroid samples (Fig. 2B, left panel).
ALCAM immunoblotting of the deglycosylated normal thyroid samples (Fig. 6B) showed a principal band corresponding to the precursor of 68 kDa.
TGFA transcripts were significantly higher in PTC metastases when compared with normal thyroid samples, and although not significant, TGFA levels trended upwards with tumor stage (Fig. 2C, left panel).
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