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Mass spectrometry analysis was used to corroborate the identity of the protein by peptide mass fingerprinting; also, the modified peptide was fragmented and sequenced by mass spectrometry, corroborating thus the inserted residues.
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This vector is independent of influenza virus-specific selection mechanisms and thus, the originally inserted sequences do not change resulting in preservation of the originally inserted HA or NA genes.
Thus the inserts settled into the sockets over time.
Thus, the insert is localised around oriC, and ranges from hsdS3 downstream of oriC to the ssl/set-locus upstream of oriC.
Thus the insert into the GvpC protein is both immune system visible and accessible.
The insert size distribution of each library was checked by Eland contained in the Illumina Pipeline, and thus the insert size range was set for SOAPaligner.
This is because the concentration of DNA in the plugs would significantly affect the methods and results of size selection on agarose gels, thus the insert sizes of the BIBAC clones resulted from the DNA [ 38].
Thus the insert usually consists of polyA, with a variable amount of upstream sequence, which may or may not include part of the L1 sequence itself (Fig. 1C) [ 18].
Thus, the researchers inserted the GABA-producing gene GAD back into an area of the brain called the subthalamic nucleus, a key regulatory center within this motor circuit, via the AAV.
Thus, the newly inserted polyadenylation signal would be expected to block any potential negative effect arising from an antisense RNA mechanism.
Thus, in both cultivars the inserted transgene is expressed in the proper organ.
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