In general, however, live animal drug screening is labor and resource intensive, and has been hampered by the lack of robust assay designs and high throughput work-flows.
Despite this, only a tiny fraction of the large number of possible solvents has been experimentally investigated, because experimental high-throughput work is complicated and costly.
For high-throughput work flows, automation, such as qRT-PCR or luciferase-based detection, might be most beneficial.
Unfortunately neither of these systems is suitable for high-throughput work, and they both provide limited flexibility in the inputs they accept.
There is a particular need for good data mining software in high-throughput work, which is a prominent part of current muscle biology research.
This is the first high-throughput work on the European tick Ixodes ricinus, which is the most important disease vector in Europe.
Whereas transient transformation of protoplasts has been proven to be highly efficient in high-throughput work flows, particle bombardment of plant cells might be preferable for investigating auxin carrier activity, because it does not affect the cell wall integrity that might be required for auxin carrier trafficking and function [ 59- 61].
Their small size and ability to grow in liquid medium makes them amenable to high-throughput work-flows using large-particle (animal) sorters, higher order microtiter plates and liquid handling robotic work-stations.
Using transgenic animals expressing fluorescently-tagged proteins, we first developed a high-quality, high-throughput work-flow utilizing an automated fluorescence microscopy platform with integrated image acquisition and data analysis modules to qualitatively assess different biological processes including, growth, tissue development, cell viability and autophagy.
