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The common biotinylated primer both reduced the cost of assay optimization and enabled high throughput second round amplification.
High throughput second generation sequencing approaches, even the benchtop platforms of which can produce up to 1.6 Gb of sequence per run [ 14], have revolutionised our approach to undertaking genetic analyses, with Genotyping by Sequencing (GBS) becoming increasingly popular.
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High throughput first-principles calculations were employed to unravel the thermodynamic stability of two sets of hierarchical phases, β"'P and β"'S, in Mg-RE (rare-earth) alloys.
Let us define the stable HP throughput first.
When the reporting delay is τ r ≠ 0 ms, the maximum average throughput first increases and then decreases as the number of cooperative SUs grows.
To calculate the throughput, first, we find the average number of transmission attempts of a packet with the limitation on their maximum number.
However, in spite of the availability of high throughput second-generation sequencers, this is still a very expensive and daunting task.
To identify differentially expressed genes from H. armigera and H. assulta, digital gene expression tag (DGE-tag) profile libraries were constructed and sequenced using high throughput second-generation sequencing technology [ 12, 13].
High-throughput second generation commercial technologies have already been developed by Illumina [ 23, 24], Roche 454 [ 25] and Biosystems/SOLiD.
To solve this problem, it is necessary to perform a high-throughput second screening of candidate sequences, although this is not possible by using the original protocol of SAGE mainly because of the very short sequence tags generated.
Jahnátek, M. et al. Ordered phases in ruthenium binary alloys from high-throughput first-principles calculations.
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