Sentence examples for throughput plate from inspiring English sources
The parametrized % quenching data for individual system shows excellent correlation with enzyme inhibition activity measured independently by standard Ellman AChE assay method in a high throughput plate reader system.
It occurred to us that substrates designed for chemiluminescent imaging (CLI) of enzyme activity using traditional high throughput plate readers could provide an alternative approach to detect lacZ gene expression in vivo.
Fortuitous parallel developments of automated systems such as liquid handling devices and high-throughput plate readers place genome-wide studies of direct biomedical relevance within immediate reach.
The assay is easily adaptable to other PR variants, a multiplex platform, as well as to high-throughput plate reader based assays and will greatly facilitate the search for novel peptide and chemical compound based PIs in T-cells.
These measurements employed high-throughput plate immunoassays to facilitate quantitative comparisons between responses to different compounds and mixtures.
We used a medium-throughput plate immunoassay to quantify phosphorylations of extracellular signal-regulated kinases (ERKs) and c-Jun-N-terminal kinases (JNKs).
TaqMan Master Mix (5 μL), 0.5-μL target gene probe and primer set, and 3.5-μL water, were added to each well of a 96-well high-throughput plate (Applied Biosystems).
(ii) Paper permeated by wax facilitates high-throughput plating of cells and resists initial spreading of aqueous solvents.
In the high-throughput plate-based assay, equimolar concentrations of proteins (400 nM) were coated on a MaxiSorp plate.
Among these 19 proteins, fucosylated hemopenxin and fetuin A were confirmed in another study by the same group in a cohort of 300 serum samples using lectin-based high-throughput plate-based assays were confirmed to have an ROC area under the curve (AUC) of 0.95 and 0.87, respectively, in the differentiation of HCC from non-HCC conditions [ 74].
Although this assay has been adequate to study the biology of these enzymes, it is not amenable to support any degree of routine inhibitor assessment because its restricted linearity is incompatible with enhanced throughput microtiter plate screening.
