These two methods offered adequate sensitivities; however they employed the expensive mass detectors that are not available in most laboratories, and involved laborious liquid-liquid sample extraction procedures that negatively affected the accuracy of the results, and limit the throughput of the procedures in screening of large number of specimens.
Nevertheless, the broad application of polysome and ribosome profiling is limited by the lack of high-throughput settings of the procedures.
In this field, indeed, robustness, high-throughput, and easiness of the procedures are the most important benchmarks.
As the demand for diagnostic testing at times of infectious disease outbreaks would be high, strategies that may enhance the throughput of analytical procedures would be advantageous.
Although the optimized vectors improved the productivity of in vitro translation, in order to build high-throughput protein synthesis systems, every step of the procedure must be accelerated, including the cloning of target genes and the purification of translated proteins.
This large contribution has resulted in optimisation and streamlining of the procedure to support the accelerated throughput required to deliver the IMPC goal in a timely manner.
However, the major limitations of the ChIA-PET technology are the complex technical aspects of the procedure, the heavy bioinformatics demand and the requirement for high-throughput sequencers.
In this study, we evaluated the feasibility of automating the serum RNA extraction procedure in order to increase the throughput of the assay.
Several cleanup procedures were tested and finally a non-classical "on batch" procedure was selected, which allows increasing the throughput of the analysis while decreasing sample manipulation.
An end-of-study survey completed by the centres indicated that only eight centres had sufficient throughput of LDI procedures during 2012 and only two centres were undertaking sufficient numbers of ALD reconstructions.
