Of these, for the LETG+HRC-S it is essential to use a source with a fairly steeply increasing flux toward low energy and with low line-of-sight ISM absorption in order to avoid the signal becoming dominated by the higher order throughput of the instrument.
The maximum safe throughput of the instrument reaches 3,000 particles per minute.
The very high throughput of the instrument is achieved by massively parallel pyrosequencing reactions, which generate a highly redundant representation of the DNA regions under scrutiny.
WGS studies are likely to become more common in the future given that the throughput of the MPS instruments continue to increase, thereby driving down the cost per base of sequencing.
While the first ChIP-seq experiments were limited by the sequencing capacity, the high throughputs of the current instruments allow many ChIP samples to be multiplexed and sequenced simultaneously in a single run.
The advancements in sequencing and genotyping technology were a prerequisite for the work described here, and further improvements in throughput of NGS instruments can be expected.
MiSeq (Illumina) generates the highest throughput per run and lowest error rate of the instruments but delivers shorter read lengths than those of the 454.
In these studies, DAE analysis was limited by the single-capillary throughput of the HRM device used, the HR-1™ instrument, and allelic imbalance was quantified manually.
The throughput of the described protocol is currently only limited by the instrument used and a larger liquid handling robot equipped with a 96-tip head could increase the throughput per run to 96 samples or more.
