Your English writing platform
Discover LudwigExact(13)
In this study, we examined the Tox21 qHTS data set generated against VDR (see materials and methods) for reproducibility and concordance in a low throughput format and investigated VDR receptor activity profiles in vitro using luciferase reporter gene assays.
Although characterization of cytoskeleton deformation has been demonstrated using several techniques like optical stretcher50, the quantitative measurement of cell nuclear deformation has not been fully studied especially in a high throughput format.
Here we describe the development of a robust and simple assay that is amenable to a high throughput format for the discovery of new antigametocyte drugs.
For this purpose, we modified the HTRF®-based Bmi1/Ring1A ubiquitination assay into high throughput format.
The samples were then analyzed directly from the 96 well plate using a C6 Flow Cytometer® System ™ (Accuri cytometers) in a high throughput format.
These results indicate that ribozymes in the tandem constructs cleave efficiently, and that the luciferase reporters are expressed from the fragmented mRNA in sufficient quantity for use in a 96-well high throughput format.
Similar(47)
A series of optimization steps for the high-throughput format were performed to minimize handling errors and to attain high well-to-well and plate-to-plate consistency.
Proteins were expressed in E. coli BL21 DE3) pLysS using a high-throughput format (0.6 ml volumes), and purified by metal-affinity capture after sonication in a buffer that contained n-dodecyl-β-D-maltopyranoside (Anatrace, Inc.).
We discuss each detection approach in terms of signal-to-background (S/B) ratio, ease of use, and adaptability to high-throughput format.
The described assay is applicable in the high-throughput format with the instrument setups designed for lanthanide-based time-gated (time-resolved) luminescence methods.
The potential use of a robust system to screen libraries of compounds in a high-throughput format, in pursuit of an inhibitor of PfHT is also described.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com