Here, we review recent advances in high throughput field phenotyping technologies designed to inform the genetics of quantitative traits, including crop yield and disease tolerance.
Araus and Cairns (2014) have reviewed advances in high throughput field-based phenotyping and highlight as an example "Shovelomics" for root traits where root numbers and their angle of growth can be assessed for a very large number of plants (Trachsel et al. 2011).
The NimbleGen 4.2 M Probe Custom DNA Microarray comes in varying formats from which we selected the highest number of plexes (12) to increase the number of samples for use in high-throughput fields studies.
Moreover, their Zea product enables high-throughput, field-based phenotyping.
However, automated high-throughput field-based plant phenotyping is now the bottleneck for scaling up such experiments.
Herein, we define key criteria, experimental approaches, equipment and data analysis tools required for robust, high-throughput field-based phenotyping (FBP).
Accelerated breeding for agriculturally relevant crop traits is key to the development of improved varieties and is critically dependent on high-resolution, high-throughput field-scale phenotyping technologies that can efficiently discriminate better performing lines within a larger population and across multiple environments.
The study was designed to determine whether the increased throughput of fields and decreased block cutting made the MLC cost effective from an institutional perspective.
We also assayed the behavior of flies from each split-GAL4 driver line in a high-throughput open-field arena described in (Kabra et al., 2013) for 15 min during dTrpA1 activation using 10X UAS-dTRPA1 (attP16) (Hamada et al., 2008) at 30°C.
High-throughput screening field will continue to be promising and dynamic in the future.
In view of the recent advancements in NMR technology [33], [34], [35], the high-throughput and/or field analysis of multiple samples in complex media should be feasible, even outside of the typical laboratory setting.
