These results indicate that substantial amounts of Cx43 are first expressed in granulosa cells following activation of follicular development and that this expression increases throughout follicular growth and maturation.
IL1A and IL1B, two proinflammatory cytokines that also initiate immune response and regulate cellular growth and differentiation, are expressed in the uterine epithelia of C. chalcides, a close relative of C. ocellatus, and this expression continues throughout pregnancy (Paulesu et al. 1995, 2005; Paulesu 1997; Romagnoli et al. 2003; Paulesu, Romagnoli, et al. 2005).
We next sought to test the validity of Ascl1, neurotensin receptor (NTR2) and NTS as cancer markers in clinical prostate material from a distinct cohort of patients by immunohistochemistry. NTS and Ascl1 were negative in around 70% of normal specimens becoming detectable in around 70% of samples on the transition to PIN and maintaining this expression level throughout subsequent Gleason grades.
The GARD analyses demonstrate that phylogenetic breakpoints occur throughout the expression sites, consistent with frequent genetic exchange.
Another two genes with homogenous expression throughout the expression array analysis were used for normalisation (table 3).
Two genes were selected as reference genes since they had homogenous expression throughout the expression array analysis.
As described throughout this review, their expression is tightly regulated, context dependent and multifactorial.
The undifferentiated human ES cell line BG01V, used throughout this study, shows NANOG expression and nuclear localization (Fig. 2A, merged image), as expected [ 38].
Note that flight muscle in male has lower expression than flight muscle in female, however inspection of the GFP-only image for male flight muscle (inset) reveals expression throughout this tissue.
Similarly, overexpression of Lhx3/4 in the posterior pole of the embryo, using the cis-regulatory sequences from Tbx6-r.b, is sufficient to cause ectopic Mesp expression throughout this territory.
