The water temperature range was 29 ± 2 °C throughout the feeding period.
Glucose concentrations fluctuated throughout the feeding period, but no treatment by time interactions were noted.
Glucose and insulin tolerance testing was conducted throughout the feeding period.
Furthermore, excretion of PFBS and PFOA via milk throughout the feeding study was very low (<1 %) and cannot explain the low affinity for muscle in dairy cows.
The numbers of spirochetes increased exponentially throughout the feeding for both WT and mutant organisms (Figure 2).
Body weight, liquid and food consumption, diarrhea and rectal bleeding (detected by eye inspection) were recorded throughout the feeding period.
In addition, there was no difference detected in voluntary feed intake between any group of mice throughout the feeding trial (data not shown).
Salivary glands and midguts were removed at different time points throughout the feeding from the WT- and bba64 mutant-infected nymphs.
WT and bba64 mutant spirochetes were visualized in the nymphal midguts at all time points (48, 57, 72 h, and repletion) throughout the feeding (Figure 3).
The medium-sized tortoises, weighing approximately 70 100 kg each, were kept together in an enclosure, where they were also being fed vegetables and other fruit throughout the feeding experiment.
