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Thus deletion of genomic sequence in the first transformation allowed mutations to be introduced efficiently throughout the deleted region.
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But before that, Grad et al. reported that Hsp90α gene trap mutant mice, in which Hsp90α is deleted throughout the entire life of the mouse, developed testicular atrophy caused by apoptosis of spermatocytes (Grad et al., 2010).
In order to study the function of procyclins throughout the life cycle we deleted all procyclin genes from a fly-transmissible strain.
For the four mutants, we predicted a total of 242 gene models to be deleted throughout the genome (Table 2).
This further demonstrates that even when Bmi-1 is deleted throughout the nervous system during fetal development, stem cells and neurogenesis persist into young adulthood and are not depleted until around a year of age.
We examined three different mouse models of Rictor loss: mice heterozygous for Rictor, mice lacking hepatic Rictor, and mice in which Rictor was inducibly deleted throughout the body in adult animals.
The most extensive intestinal hyperproliferation and adenoma formation resulted from Cre-mediated excision of exon 3 (encoding amino acids 5-80) of the endogenous ctnnb1 gene throughout the intestinal mucosa, thereby deleting all the regulatory serine and threonine residues that control the turnover of the β-catenin protein (Harada et al., 1999; Leedham et al., 2013).
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