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All mice were created in the FVB-C3H/B6 background and remained in a consistent background throughout breeding.
In a two-cohort study design, 48 females (24/cohort) were assigned to parallel exposure groups at 0 (control), 200, 600, or 1800 ppm methanol vapor for approximately 2.5 h/day, 7 days/week throughout breeding and pregnancy.
To induce H2BGFP expression, breeders and pups were treated with doxycycline throughout breeding, pregnancy, lactation, and after weaning until sacrifice.
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However, there were relatively small differences in corticosterone levels among years, but levels declined throughout each breeding season, which was opposite our predictions for stress hormones correlating with a declining population.
They enlisted volunteers to hand-rear caterpillars on milkweed at sites throughout their breeding grounds, then compared the carbon and hydrogen in the wings of the adult butterflies to those gathered from 597 dead adults at the winter roosts.
This continues throughout their breeding lives, but the biggest increase is in the first five years.
DA females were used throughout the breeding program to ensure that mitochondrial DNA was inherited from DA.
To monitor the 15N incorporation throughout the breeding scheme, 15N incorporation rates were calculated for PND5, 14, 28 and 56 in brain and plasma samples.
Nests were then monitored throughout the breeding season to collect data on laying date, clutch size, nestling weight and fledging success.
Our results demonstrate that Laysan albatrosses are able to assess prey availability over large scales and make foraging decisions based on the energy required to reach feeding grounds throughout their breeding cycle.
All nestboxes were monitored throughout the breeding season to determine the onset of egg laying (March 1st = day 1), clutch size, hatching date, the number of hatchlings, and the number of chicks fledged.
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