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Group housed C57Bl/6J 9 weeks old male mice underwent stereotactic surgery, after which they were singly-housed throughout all subsequent testing.
Nine-week-old male C57Bl/6J mice were group housed until they underwent stereotaxic surgery, after which they were singly housed throughout all subsequent testing, at a constant temperature (22 ± 1°C) and 12-h light/dark cycles.
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All subsequent testing was conducted in these cages.
All isolates were stored at −80°C for subsequent testing.
Subsequent testing revealed hemagglutination activity in all 6 specimens.
This testing technique was retained throughout all subsequent experiments.
After review and retraining, all readers demonstrated acceptable reproducibility, which was maintained on subsequent testing 1 year later.
The flukes placed in the control wells remained alive and healthy throughout all the tests.
Another important feature of ESSE is that the initial sampling properties have been maintained throughout all subsequent years.
A mean NGF volume is calculated from a sample image and used throughout all subsequent calculations.
Unless stated otherwise, this concentration was used throughout all subsequent experiments.
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