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The minimal requirement for the IFNB1 S/MAR to function in DNA replication and episomal retention is transcription through this element.
However neither overexpression of NRF-1 nor expression of a dominant negative mutant nor NRF-1 RNAi significantly affected miR-22 promoter activity (Fig. S2), suggesting that a transcription factor other than NRF-1 activates the miR-22 promoter through this element.
Through this element, p21 interacts with cyclin cdk complexes, and interacts with components of the receptor trafficking and endosome sorting machinery.
The T-to-C mutation of the Crh− 120 /+ mice resulted in an increase in promoter transcription in the reporter assay, thereby indicating that the mutation either prevented inhibition through this element or acted positively to enhance transcription factor binding and activity; either possibility would result in increased transcription and thus effectively a gain-of-function mutation.
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Small variations in mass-dependent fractionation of multiple sulfur isotopes were proposed to result from complex mass-flow of this element through enzymatic systems [48].
The ability to document Cd exposure and uptake of this element through biological monitoring is a first step towards understanding its health effects.
Militello et al. (2004) computationally predicted a cis-regulatory element in the upstream sequences of 8/18 P. falciparum heat shock genes (called the G-Box) and subsequently demonstrated the importance of this element through transient transfections and mutational analyses [ 15].
The 'Hands up for Max!' intervention had a homework element with the potential to effect change in the wider community through involving parents, but this element was optional and inconsistently used even within the trial.
The TxRE consists of a cyclic AMP-response element (CRE -like sequenCRE -likeax hasequenceuggested to bind indirectly to this element through cellulandfacTaxs, suchass membeen of the CREB/activating transuggested factor (ATF) family of bindc leucindirectly protoins this havelementshown through to the cellular sequence [ 5- 7].
Through this interfacial element approach, VCCT can be implemented into a commercial finite element analysis (FEA) code having user subroutines without interrupting the main code.
While we cannot rule out the possibility that another promiscuous homeodomain protein regulates the expression of RdCVF through this "CRX" element, CRX it is the most likely candidate to be mediating the observed biological activity.
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