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The interviewer teams were walked through the objectives, methods and tools of the study to enable them to grasp the details of the study requirements.
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Through-the-objective total internal reflection (TIR) occurs at the coverslip/aqueous interface where a HCRLC-PAGFP exchanged muscle fiber in aqueous buffer solution contacts the coverslip and is illuminated by the evanescent field.
More recently, commercial equipments were made available with through-the-objective illumination.
In vitro motility was observed with through-the-objective total internal reflection fluorescence (TIRF) on an Olympus IX71 inverted microscope using a 150×, 1.45 NA objective.
As previously emphasized, 26 the illumination intensity on defocused points is not exactly described by a single exponential, particularly when through-the-objective illumination is performed.
Imaging was carried out using an OLYMPUS inverted microscope with a 100×/1.49 numerical aperture TIRF objective, equipped with through-the-objective TIRF illumination, a 37 °C temperature-controlled stage and an objective warmer (Live Cell Instrument, Seoul, Korea).
Light shines down through the objective and is focused through the objective onto the specimen.
Lee Silberstein, a spokesman for AKRF, said in a statement: "For more than 25 years, AKRF has built its reputation through the objective gathering and analysis of data.
If we view him through the objective lens of science, we could at least try to determine the cause of these acts of violence that seem to be proliferating in our country.
The laser light was focused through the objective (×20) of an Olympus BX-41 microscope.
Tolerance to error was evaluated through the objective tasks completion scenarios.
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