The genome and its annotation are available through the Integrated Microbial Genomes (IMG) portal (Markowitz et al. 2012) at https://img.jgi.doe.gov/, along with auxiliary data such as clustering of genes into paralogous groups and their assignment into functional categories (based on matches to COG, Pfam, TIGRfam, and InterPro databases).
Finally, the above predictions and annotations were reconciled with the genes predicted and annotated through the Integrated Microbial Genomes Expert Review (IMG-ER) pipeline [ 124].
The initial assembly has also been uploaded and can be accessed and utilized for future analyses and studies through the JGI's IMG system using the IMG Genome ID 642555144 (https://img.jgi.doe.gov/cgi-bin/m/main.cgi section=TaxonDetail&page=tax onDetail&taxon_oid=642555144).gov/cgi-bin/m/main.cgi section=
Detailed exploration of the gene context data using these tools is available through the framework of IMG.
Annotations corresponding to known dockerin (IPR01824, IPR016134, IPR002195) and cohesin (IPR002102 and IPR018452) domains were obtained from Interpro [ 56] through the DOE JGI IMG database [ 57].
The analysis was further limited to the single M. maripaludis, Sulfolobus islandicus, and S. solfataricus cultivars listed in Figures 2, 3 and Additional File 1. Except where otherwise noted, gene candidates were identified using the BLAST search tool provided through the Joint Genome Institute's IMG site http://img.jgi.doe.gov, with results limited to the selected fully-sequenced archaea.
Someday, the parents who shell out as much as $60,000 a year for Nick's Junior Accelerated Full-Time program -- operated through the agent hands at IMG, proudly posting all programs and prices on its Web site -- will wake up and see the trend: tennis factories are so 1990's.
If anything, the shows may become more action-oriented, more entertainment-driven, if a proposed sale of 7th on Sixth, the nonprofit group formed by the designers council to run the shows, to IMG goes through.
This was done through the
Explicitly, given the repression level for the system with a single operator (Rnoloop), the repression level for two operators with looping follows from the free energy of looping through the
