On the left side of the C6 segment, 1.5 µl of viral stock was manually injected through sharpened microcapillary glass (PN-30 puller, Narishige) connected via silicone to a 10-µl Hamilton microsyringe at a rate of 0.5 µl/min.
As future programming deepens and widens, good programming skills, child participation, and monitoring and evaluation are areas that require refinements through sharpened programming skills and focus on child well-being-related results.
The bite of this powerful moral fable is sharpened through listening rather than reading.
When snow blankets the details of a familiar world, perceptions, particularly of space, and of moving through it, are sharpened.
Despite Keane's frequent depiction as a lunatic, these were not mindless rantings, but coherent thoughts, honed, refined, ordered and sharpened through a period of a years and body of evidence.
This time, Germany has taken the opportunity to use the less than forthright accounting methods of the Greek government to say that it would not directly rescue Greece, that the provisions for control and sanction in the rules governing the euro zones must be sharpened through possible revisions in the Lisbon Treaty, the quasi-E.U.
Then comes the extraction of the lignite itself, a giant wheel grinding through the seam, sharpened scoops each carrying the weight of a small car in coal.
He argues that for artists, "focus and endurance" are almost as important as talent — and far more achievable, "since they can be acquired and sharpened through training".
Rebeck, whose comedy "Seminar" was recently at the Ahmanson Theatre, is a prolific playwright with a facility, no doubt sharpened through her years as a TV writer and producer, for cooking up semi-satiric dramatic situations, seasoning them with up-to-the-minute social minutiae and concluding with a dollop of cautionary wisdom.
Lot of ideas can be sharpened through discussions with more and more people.
The O2 deal would then set the stage for even more consolidation.If the Telefónica-O2 deal goes through, it will sharpen competition in the mobile market.
