Sentence examples for through serial steps from inspiring English sources
An aliquot of 3 μg total RNA in 100 μl diethyl pyrocarbonate (DEPC) water was processed through serial steps of heating at 65 °C for 5 min, cooling on ice for 5 min, incubating for 1 h in a solution at 4 °C, and purification by Microcon 30 before RT-PCR.
Variation in conductivity of the final suspension was minimized through serial washing steps before each assay (See Text S1 for additional discussion on medium conductivity and Table S1 for individual cohort solution conductivities).. Second, the OET-compatible mediusedsed here (EP) has not been optimized for compatibility with embryos.
After addition of MgCl2 to a concentration of 5 mM, a concentration kept through-out the purification process, the dialysed extract was centrifuged once more, and this final extract was subjected to serial steps of chromatography with adequate dialyses between each step (all steps performed at 12°C).
Both target and reference DNA standards were diluted in 8 to12 serial steps, each applied in duplicate.
For double antibody staining, sections were incubated with the two antibodies in two serial steps.
Recent findings reveal that lncRNAs are implicated in serial steps of cancer development [ 113].
The bacterial suspensions were diluted in tenfold serial steps up to the 10−6 dilution stage.
The α-linolenic acid, a nutritionally essential fatty acid that must be obtained through the diet, can be converted in the vertebrate liver or brain to docosahexaenoic acid (DHA, C22 6n-3 C22 6n-3a serial steps of desaturation and elongation with final peroxisomal chain shortening [ 3], by means of desaturases and elongases [ 4, 5].
The library was screened by quantitative PCR (qPCR) through successive steps using BAC super-pools and pools, serial dilutions and clone identify verification by amplicon sequencing.
The final diagnosis had always been done by a meticulous examination of serial step sections.
Li played her part, smiling through serial bouts of adversity.
