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This was verified through ratio calculation using the Soprano software.
Through ratio optimization of the MUC1 and HER2 aptamers, the binding capacities of the Dual-SiNPs to both cell lines were maximized.
Ensemble FRET efficiency (E) values were obtained through (ratio)A analysis (38) and are the average from two experiments.
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In the US market, the "sell-through" ratio is about 60%, but it can go as low as 30% on some markets.
(LinkExchange, the web's first ad network, coined the acronym "CTR" for Click-Through Ratio – more on that in a bit).
Only the bleed-through ratio needs to be determined in a calibration measurement.
Cross-talk correction is simple, but it is restricted by combinations of the values of the bleed-through ratio, the brightness ratio, and the number ratio.
The precise determination of the bleed-through ratio κ is important for any cross-talk correction and should be performed as a calibration measurement during each measurement session on a particular system of labeled molecules and optical setup.
The cross-correlation caused by cross-talk, x κ /g, is given by the product of the bleed-through ratio κ and the ratio f of the apparent (i.e. measured) count rates in the green and the red channel.
The bleed-through ratio κ= (where denotes the brightness of the Green dye in the green channel and the brightness of the Green dye in the red channel) is determined from a one-time calibration measurement.
This ratio is denoted as the bleed-through ratio of the Green dye to the red channel, and of the Red dye to the green channel (see Table 1 for an overview of parameters).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com