Sentence examples for through pulldown from inspiring English sources

However, although Crk has been well characterized upstream of Rac [ 34], we do not observe a strong correlation between Crk knockdown and overall Rac GTP levels, as assessed through pulldown assays.

These protein components have been established through pulldowns of overexpressed epitope tagged subunits or by using antibodies raised against specific subunits.

The folding stability of variants produced in this way have been assessed by their susceptibility to limited proteolysis, and the phosphopeptide binding activities of the variants have been assessed through phosphopeptide pulldown studies.

Biotin-containing fragments were enriched through streptavidin bead pulldown before PCR amplification of the library.

PD: pulldown; FT: flow through; Control: non-crosslinked sample; UVC: short wavelength UV.

To evaluate whether LoNA binds to NCL through these binding motifs, we performed pulldown of biotinylated RNA and found that LoNA sequences harboring mutations M1 or M1 + M2 exhibited substantially weaker binding affinity to NCL compared to WT LoNA, whereas LoNA harboring mutations M2 had similar binding affinity to WT (Fig. 3f).

The GUI makes programming the device fairly simple through the use of the tabbed organization and simple pulldown menus, as well as the inclusion of a medication library.

Using pulldown assays, we demonstrated that SOCS1 interacted with both ATM and ATR through its SOCS box [ 34].

On the right side, experimental steps of the pulldown experiment are shown (I = Input; FT1 = flow through 1; FT2 = flow through 2; E = elute of bound proteins).

Choose the pulldown and choose Volume Roughing.

Liposome pulldown assays were performed as described previously68.