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To avoid sectioning through nuclei, 10 µm sections were prepared and placed on glass slides as described above for immunohistochemical analyses.
Briefly, a Zeiss Axio Imager with a 100× Zeiss alpha Plan-Fluar objective (NA = 1.45) and a Hamamatsu ORCA-ER digital camera were used to capture 19-image stacks of 170-nm step size through nuclei of log-phase cells at room temperature.
Deconvolution, maximum intensity projections, area measurements, and linescans across 0.5 µm confocal sections through nuclei incubated with fluorescein-labelled dextran were made using Metamorph (Universal Imaging), 3D volumes were rendered using Volocity 4 (Improvision), and Photoshop 7.0 was used to pseudocolour and merge images and for assembly of Figures.
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Moreover, it was suggested that migraine triggers cause cortical activation, which disinhibits trigeminovascular sensation through brainstem nuclei (NRM) involving a serotonergic mechanisms.
The AxioVision 4.1 image acquisition software was used to obtain optical sections at 0.85 µm intervals through selected nuclei.
TB transmission occurs through droplet nuclei aerosolized by patients with infectious pulmonary TB and inhaled by other persons.
Optical sections through the nuclei were captured at 0.5 μm intervals, and images were obtained by projection of the individual sections.
Shown are Z-projected confocal sections through pachytene nuclei (A – D ), the distal region of the gonad (E ), and entire dissected gonads (F ).
The minutiae density was determined within a circle whose diameter cuts 18 ridges and passing through the nucleus of the fingerprints.
The minutiae density was determined within a circle whose diameter cuts 18 ridges, and passing through the nucleus of the fingerprints as described in the previous methods (Okajima 1970; Adamu et al. 2017).
The objectives of this work include automatic recovery and visualization of a 3D chromosome structure from a sequence of 2D tomographic reconstruction images taken through the nucleus of a cell.
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