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Unlike acutely transforming retroviruses, these retroviruses do not disrupt normal cellular functioning through insertion of a viral oncogene.
Results show that lengthening of luciferase domains through insertion of a flexible linker did not affect bioluminescence emission spectra.
In Drosophila melanogaster, yellow has been implicated in melanin pattern formation on larval mouth parts and adult wings, mediated either through insertion of a transposable element closely downstream of yellow [32] or by a wing-specific cis-regulatory element [10].
During the course of creation of our mouse models and analysis of the phenotypes, it has been reported that homozygous knockout of Grp94 through insertion of a neo resistance cassette into the coding region at the end of exon 3 (61 amino acids into the mature protein) of the murine Grp94 gene leads to embryonic lethality around day 7 of gestation [34].
The ΔgL virus was produced from the K181-Perth strain of MCMV, through insertion of a lacZ expression cassette under the control of the HCMV IE promoter by homologous recombination into the gL ORF as described elsewhere [(Allan et. al., manuscript submitted) and [30]].
After local anesthesia with 2% lidocaine the vein puncture was performed through insertion of a 7-French sheath in Seldinger technique.
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Most typically, this involves the use of computed tomography (CT) or magnetic resonance (MR) imaging, and in many cases, an invasive intra-cranial pressure (ICP) monitoring through insertion of an intracerebral catheter.
On the other hand, Duane et al. demonstrated a greater risk of UTI with bladder pressure measurements using an open technique in which the Foley catheter was compromised through insertion of an 18-gauge needle and disconnection of the Foley catheter to allow instillation of 50 ml of saline into the bladder [14].
(191) The identification of SUMOylation sites is furthermore facilitated by introducing an artificial tryptic site through insertion of an arginine residue.
On the other hand, Duane et al. demonstrated a greater risk of UTI with bladder pressure measurements using an open technique in which the Foley catheter was compromised through insertion of an 18-gauge needle and disconnection of the Foley catheter to allow instillation of 50 ml of saline into the bladder [ 14].
Another evolutionary trend is the formation of a barrel-like configuration through insertion of strands which on instances provides a classical interaction interface at the open end of the barrel.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com