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Surface-bound radioactivity was removed from the cells through incubating the cells twice with 0.5 mL glycine-HCl in PBS (50 mM, pH 2.5) for 5 min at 37 °C.
After the sections were dewaxed, antigen retrieval was achieved through incubating in H2Od containing 50 mM sodium citrate (pH 9) for 15 minutes at 80°C, followed by washes in 0,1M phosphate-buffered saline (PBS; pH 7.4).
First, through incubating CPV capsids with SAM, GTP, ATP, and Mg2+, we obtained the SGA-CPV particle which, lacking of UTP and CTP, is incapable of mRNA transcription.
The in vitro release of protein was studied for 21 days through incubating 30 35 mg of nanofibers in 5 mL of PBS in a 15 mL conical tube.
The ability of Lactobacillus isolates to prevent Salmonella typhi adherence and invasion was examined through incubating a mixed suspension of tested Lactobacillus isolate and Salmonella typhi isolate SS6 with Vero cells for two hours.
The purpose of this study was to characterize the nature of DNA reactive chemicals in foundry air samples through incubating the foundry filter extract with DNA and activation enzymes.
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The flow-through was incubated again with new CD44 antibody-coated magnetic beads to obtain a pure CD44low fraction.
These factors raise the likelihood of importation either through travelers incubating plague (as occurred in New York 2002 [ 7 ]), or through importation of infected vectors, such as fleas or rats.
Many of our greatest scientific achievements have been incubated through long term strategic government investment in our world-class universities.
Africa Internet Holding currently employs more than 1,500 staff through its incubated startups.
Additionally, she has served on a number of advisory boards, including BlogHer and PopTech, a non-profit that catalyzes social impact through programs, incubated initiatives, salons and an annual conference, among others.
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