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A significant number of introns have been validated through expressed sequence tag (EST) comparisons.
High throughput technologies through expressed sequence tag cDNA/ETS/mRNA/CAGE sequences have been developed that can identify TSSs with high accuracy [ 30, 62, 63].
Transcriptome analysis has been a major driver in the comprehensive molecular characterisation of cancer through expressed sequence tag sequencing, as well as tag-based approaches including SAGE.
To date, neuropeptides that have been shown to directly modulate immune function through expressed receptors include SP, the pancreatic polypeptide family (including NPY), calcitonin gene-related peptide (CGRP, VIPP and gastrin-releasing peptide (GRP).
To identify a priori the specific molecular mechanisms of those contributions it is necessary to consider the mycorrhizal metabolome model as a complete network of compartmentalized metabolisms interacting by transported metabolites through expressed membrane transporters.
Recently two new TNF ligands were discovered through expressed sequence tag (EST) database searches: a proliferation-inducing ligand (APRIL or CD256, TNFSF13) [ 13- 15] and B lymphocyte stimulator (BLyS), which is also known as B-cell activating factor of the TNF family (BAFF, also reported as TALL-1, CD257 and TNFSF13B) [ 15- 18].
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