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Cartilage tissue engineering through culturing chondrocytes in 3D porous scaffolds has potential for the repair of articular cartilage defects.
The ECM was obtained through culturing the hBMSC on a β-TCP porous scaffold and performing decellularization and sterilization.
Our results showed that MADM microcarriers retained the ultrastructure of the acellular dermal matrix, had good biocompatibility, and supported human fibroblast expansion either as a direct culture substrate or through culturing cells in conditioned medium prepared from them.
To develop a novel scaffolding method for the copolymers poly lactide-co-glycolide acid (PLGA) to construct a three-dimensional (3-D) scaffold and explore its biocompatibility through culturing Schwann cells (SCs) on it.
After MC3T3-E1 cells had achieved different stages of OB differentiation through culturing with or without BMP-2 and/or SB431542 for 3, 6 and 9 days, they were washed and subsequently cocultured with 5TGM1 MM cells for 3 days.
Bone marrow cells were allowed to differentiate into DCs through culturing in RPMI (Sigma-Aldrich, St . Louis USA) supplemented with 10% Fetal Calf Serum (FCS) (Cultilab, Brasil), 10 mg/mL gentamicin (Schering-Plough, USA), and recombinant cytokine GM-CSF (50 ng/mL) (Peprotech, Brasil) for 7 days.
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(See my previous post about the risks of leading through culture).
Amorphous aluminosilicates with increased A/Si ratio were found in the anaerobic, live flow-through culture as well as in the seven days incubation of aerobic live culture.
Culturally we changed ourselves too, developing new capacities needs and desires through culture and civilisation.
It is happening through culture.
Strength through culture is not just words.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com