Sentence examples for through containing the from inspiring English sources

For mass spectrometry analysis, the flow through containing the N-terminal cleavage product was concentrated to 200 µL and precipitated at −20°C with 1 mL of 10% Trichloroacetic acid (TCA) in acetone.

After centrifugation to pellet the aggregates the supernatant containing S-HDL-SAA fraction was applied to the column pre-equibrated with 25 mM sodium acetate, 125 mM NaCl, pH 5.2 and the flow through containing the S-HDL-SAA was collected with heparin remaining bound to the column.

The flow through containing the glycopeptides were collected by centrifugation and purified by C-18 chromatography.

The re-loading was performed at 4°C overnight with gentle shaking, and then the flow through containing the released Rv0011c was collected and analyzed by SDS-PAGE.

If necessary, use your hands to pull back on the rear derailleur (the "arm" that the chain passes through containing the small pulleys) and/or maneuver the chain out of the way as you remove the wheel.

The cleaved protein was purified from the protease and the cleaved affinity tag by passing the cleavage product over the nickel-affinity column and collecting the column flow-through containing the purified protein.

The flow-through containing the TEV-treated protein was collected.

The flow-through containing the protein was pooled and applied to a Hitrap SP column equilibrated with the same buffer.

The flow-through containing the protein was pooled and applied to a Hitrap Heparin and SP column (Amersham Biosciences) equilibrated with the same buffer.

The flow-through, containing the native enzyme, was concentrated and diluted in ESB as described above and the proteins were analysed by SDS-PAGE.

Protein solutions were reapplied to a HiTrap Ni2+ FastFlow column (5 mL) (G.E. Healthcare, QC, Canada), and the flow-through, containing the cleaved IC3 or IC3-C58 proteins, was collected.