Sentence examples for through containing from inspiring English sources

The flow through containing tag-less MtbHadAB complex was collected, exchanged in buffer containing 50 mmol/L Tris-HCl, pH 8.0, 50 mmol/L NaCl, and 10% (v/v) glycerol, and applied to a Resource Q anion exchange column (GE Healthcare) equilibrated with the same buffer.

The flow through containing p11 was dialyzed against PBS containing 1 mM DTT and concentrated with Centricon 3 (Amicon), and stored at −20°C in aliquots.

For mass spectrometry analysis, the flow through containing the N-terminal cleavage product was concentrated to 200 µL and precipitated at −20°C with 1 mL of 10% Trichloroacetic acid (TCA) in acetone.

After centrifugation to pellet the aggregates the supernatant containing S-HDL-SAA fraction was applied to the column pre-equibrated with 25 mM sodium acetate, 125 mM NaCl, pH 5.2 and the flow through containing the S-HDL-SAA was collected with heparin remaining bound to the column.

The flow through containing the glycopeptides were collected by centrifugation and purified by C-18 chromatography.

The flow through containing FPH4 was purified using a phenyl-Sepharose column, followed by a polishing step via size exclusion chromatography (GE Healthcare).

The cleaved protein was purified from the protease and the cleaved affinity tag by passing the cleavage product over the nickel-affinity column and collecting the column flow-through containing the purified protein.

The flow-through containing unbound protein was discarded and a stepwise NaCl elution was used to elute the correctly folded dimer at 200 mM NaCl (H1) and the majority of soluble aggregates at 600 mM NaCl (H2).

The flow-through containing the TEV-treated protein was collected.

The flow-through (containing pure hArc protein, either WT or V231G hArc variant) was collected.

The flow-through containing the protein was pooled and applied to a Hitrap SP column equilibrated with the same buffer.