Sentence examples for through contained the from inspiring English sources
The flow through contained the purified oligonucleotides.
The solution that passed through contained the plant hormones.
The flow-through contained the purified CD34+ fraction.
The cleaved protein was purified from the protease and the cleaved affinity tag by passing the cleavage product over the nickel-affinity column and collecting the column flow-through containing the purified protein.
The flow-through containing the TEV-treated protein was collected.
The flow-through containing the protein was pooled and applied to a Hitrap SP column equilibrated with the same buffer.
The flow-through containing the protein was pooled and applied to a Hitrap Heparin and SP column (Amersham Biosciences) equilibrated with the same buffer.
The flow-through, containing the native enzyme, was concentrated and diluted in ESB as described above and the proteins were analysed by SDS-PAGE.
Protein solutions were reapplied to a HiTrap Ni2+ FastFlow column (5 mL) (G.E. Healthcare, QC, Canada), and the flow-through, containing the cleaved IC3 or IC3-C58 proteins, was collected.
The flow-through, containing the low-abundance proteins, was collected, and the high-abundance proteins that remained bound to the column were eluted with buffer B at a flow rate of 1 ml/min (buffers A and B are proprietary buffers included with the MARS depletion kit, and their composition is not disclosed by the manufacturer).
For mass spectrometry analysis, the flow through containing the N-terminal cleavage product was concentrated to 200 µL and precipitated at −20°C with 1 mL of 10% Trichloroacetic acid (TCA) in acetone.
