That suggested they formed from interactions between bits of the backbones, rather than the variable side chains.Dr Dobson then went a step further and showed that even proteins that had nothing to do with disease could form amyloid through backbone interactions.
The electric energy transmission business, transmits electricity from large generators through backbone grid lines to distribution grids which supply power to end consumers.
Structural analysis in a membrane revealed that all extracellular loops of rhomboid make stabilizing interactions with substrate, but mainly through backbone interactions, explaining rhomboid's broad sequence selectivity.
To assess the contribution of large side chains to α-helix stability through backbone desolvation and salt-bridge formation, we simulate the alanine-rich peptide, Ac-YAEAAKAAEAAKAAEAAKAF-Nme, referred to as the EK peptide, that has three pairs of "i, i + 3" glutamic acid and lysine substitutions.
The glycyl radical of class III RNR is sensitive to oxygen; exposure results in enzyme destruction through backbone cleavage.
However, the rest of the sequence in this region is poorly conserved as most other interactions occur through backbone oxygen or nitrogen atoms [ 10].
UDP is tethered through backbone hydrogen bonds, a hydrogen bond to the ribose and several basic residues interacting with the phosphates.
During the process of CP, the native N- and C-termini of a protein are covalently linked with a short peptide, and new termini are introduced through backbone cleavage elsewhere in the polypeptide sequence.
Notably, both the backbone and side-chain of N736 in PfLAMMER are involved in hydrogen bonding to the backbone of the catalytically important HTD motif, while in other CMGC kinases, the coupling between the P+1 pocket and catalytic site is largely mediated through backbone hydrogen bonds.
The analysis of the carboxin-bound structure (2WDQ) suggests an alternate path through backbone covalent bonds of Cys-206 and His-207 of SdhB, then a through-space jump from the CD2 atom of the imidazole ring of His-207, and then on to carboxin (path 3, Figure 2).
