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Each partition step results from a single cut through all sequences, so clusterings are conflict-free at each step of the procedure.
If sufficient number of sequences are given and the motif model is not highly degenerate, i.e. small d with respect to l, the likelihood that an l-sized motif can span through all sequences by chance is rare.
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We can therefore calculate the entire set of orderings for all k in a single pass through all the sequences, in time proportional to NM.
After scanning through all sequence reads, the alignment tool generates a file containing the collected information.
We ran the sequences through all the programs and found that iHMMune-align selected 47/57 identical rearrangements for the first group of sequences, while SoDA2 selected 34/57 identical rearrangements.
Thus, the candidate sequences through all the analyses above are considered as HTGs between bacteria and insect.
The smaller value (3,405) comes from the PHYTOPROT project [ 34] and were obtained through extensive all-against-all sequence comparisons using the LASSAP software [ 36].
The separation can be performed using from conventional to fully thermally coupled distillation sequences going through all the intermediate possibilities of partial thermal integration.
The input data was prepared by collecting proteins in the release 50.9 of UniProtKB/Swiss-Prot (235673 entries), and the discovered patterns were verified through all protein sequences in the release 2010/08 of UniProtKB/Swiss-Port (518415 entries).
Because not all sequences extended through the first and ninth variable regions, I further screened these sequences to generate a collection of 139,987 candidate sequences for analyzing the V12 and V14 datasets and a collection of 77,685 sequences for the V89 and V9 datasets.
All sequences found through this search plus the three Arabidopsis sequences were used to generate a Hidden Markov Model (HMM) profile to iteratively search the same genomes (see Methods).
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